Noninvasive Detection of Alpha-Amylase in Saliva Using Screen-Printed Carbon Electrodes: A Promising Biomarker for Clinical Oral Diagnostics

Universitas Padjadjaran Develops Innovative Noninvasive Saliva-Based Diagnostic Tool for Oral Health

Researchers from Universitas Padjadjaran, including Maria Komariah, S.Kp.,M.Kes., Ph.D. and colleagues from the Faculties of Dentistry, Mathematics and Natural Sciences, Nursing, and Geological Engineering, in collaboration with Universitas Mulawarman, have successfully developed a novel, noninvasive method to detect alpha-amylase, a key biomarker for oral health, using advanced screen-printed carbon electrodes (SPEs). This breakthrough offers a faster, more comfortable, and cost-effective diagnostic alternative by using saliva as a biological source.

The study employed electrochemical techniques, Cyclic Voltammetry (CV) and Differential Pulse Voltammetry (DPV), to analyze saliva samples using gold nanoparticle-modified SPEs. Results showed a strong linear correlation (R² = 0.9513) between voltammogram responses and alpha-amylase concentrations (100–500 units), with a detection limit of 104.252 units and a quantification limit of 315.915 units, making it highly sensitive for clinical applications.

By advancing low-cost, noninvasive biosensor technology, this research supports SDG 3 (Good Health and Well-being) and SDG 9 (Industry, Innovation, and Infrastructure) through accessible and accurate diagnostic solutions for oral health.

For further details, read the full article published in Medical Devices: Evidence and Research:
https://doi.org/10.2147/MDER.S493383